• Grading: literature reading, translation & review 40%; Presentation 50%; Class attendance 10%

1. A brief history of synthetic biology （Nature Reviews Microbiology 2014）
   
2. Construction of a genetic toggle switch in Escherichia coli（Nature 2000) 15
3. A synthetic oscillatory network of transcriptional regulators（Nature 2000）16
4. Engineering Escherichia coli to see light（Nature 2005) 40
5. A synchronized quorum of genetic clocks（Nature 2010) 18
6. ...(related articles)
7. Synchronized bacterial clocks（Nature 2010)
8. The yin and yang of nature（Nature 2009)
9. Ten years of synergy（Nature 2010)
10. Five hard truths for synthetic biology（Nature 2010)
11. Programmed population control by cell–cell communication and regulated killing（Nature 2004)
12. A synthetic multicellular system for programmed pattern formation（Nature 2005)
13. A synthetic gene–metabolic oscillator（Nature 2005)
14. A bottom-up approach to gene regulation（Nature 2006)
15. Production of the antimalarial drug precursor artemisinic acid in engineered yeast（Nature 2006)
16. Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors（Nature 2007)
17. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels（Nature 2008)
18. Evolvability and hierarchy in rewired bacterial gene networks（Nature 2008)
19. A fast, robust and tunable synthetic gene oscillator（Nature 2008)
20. A tunable synthetic mammalian oscillator（Nature 2009)
21. Programming cells by multiplex genome engineering and accelerated evolution（Nature 2009)
22. Spatiotemporal control of cell signalling using a light-switchable protein interaction（Nature 2009)
23. Large-scale de novo DNA synthesis: technologies and applications（Nature Methods 2014)
24. Synthetic biology: back to the basics（Nature Methods 2014)
25. A synthetic approach to abiogenesis（Nature Methods 2014)
26. Programming biological operating systems: genome design, assembly and activation（Nature Methods 2014)
27. Principles of genetic circuit design（Nature Methods 2014)
28. Interactive assembly algorithms for molecular cloning（Nature Methods 2014)
29. Synthetic Biology Edition (Nature Biotechnology 2009)
30. Latest published Nature articles... (Click to access)
• Josefine Liljeruhm, Erik Gullberg, Anthony C.Forster. Synthetic Biology: A Lab Manual. World Scientific, 2014.(book)
• New life in the laboratory: Can synthetic biology bring sustainable development to China? （The Biochemical Society 2011）
• 合成生物学： 学科基础、研究进展与前景展望（前沿科学2007）
• 基于文献计量学的世界合成生物学研究发展趋势及展望（中国基础研究2013）

• Introduction (clip)
• Doing Things with Living Organisms (clip)
• The Construction Plan (clip)
• A Complex Matter (clip)
• Applying Engineering Principles to Biology (clip)
• The Minimal Organism (clip)
• Standard Biological Parts (clip)
• Printing Life (clip)
• From DNA to XNA (clip)
• Protocells: Creating an Artificial Cell (clip)
• The Benefits (clip)
• Open Source Biology (clip)
• Synthetic Biology Ltd. (clip)
• Killer Applications (clip)
• A Future Technology (clip)

• Minimal genome (Wikipedia)
• M. genitalium
• E. coli

• Genome editing (Wikipedia)
• ZFN(zinc-finger nucleases)锌指核酸内切酶
• TALEN(transcription activator-like effector nucleases)类转录激活因子效应物核酸酶
• CRISPR/Cas9（Clustered Regularly Interspersed Short Palindromic Repeats）基因组定点编辑技术
• NgAgo-gDNA基因编辑新技术

• The Synthetic Biology Open Language (SBOL) provides a community standard for communicating designs in synthetic biology （PDF） （Video）
• SBOL Visual: A Graphical Language for Genetic Designs (PDF)
• 
• Regstry of Standard Biological Parts （Lab Techniques）（PPT）

Bio-enery, Marine biotechnology, ...

• Introduction of Synthetic Biology (Foundations of Engnieering Biology)
• DNA and Genome
• Genetic Circuits (Reconstruction of Genetic Circuits)
• BioBricks (BioBricks Website)
• RNA (RNA in Synthetic Biology)
• Protein Engineering
• Metabolic Engineering
• Ethics and Business