About CH-ATAC Landscape
We used CH-ATAC-seq to profile chromatin accessibility landscapes in zebrafish, Drosophila and earthworm. To allow for public access of the resource, we created a CH-ATAC Landscape website at http://bis.zju.edu.cn/chatac/.
CH-ATAC Landscape website consists of seven web pages.
1. Home: This page contains three sections. About describes the functions and update of the CH-ATAC Landscape website, workflow gives a brief description of the sequencing method used in the project, CH-ATAC-seq.
2. Atlas: Explore cell clusters in different datasets. Users can view each cluster, each tissue and each gene by choosing the optional box on the left. Atlas view provides global view on single cell level, Marker list provides marker genes for each cluster.
3. Celltype: Explore detailed information for every cell type in different datasets.
4. Track: Load signal tracks and compare them among cell types/regions in different datasets.
5. Download: This page provide all the datasets information used in this project.
6. FAQ: Some Frequently Asked Questions are listed on this page, inlcuding the corresponding dataset and code address.
7. Contact: Contact information for data providers are placed in this page. We also provide message board on this page.
News
On May 08, 2023: CH-ATAC Landscape released.
Step 1. Nuclei extraction.
Step 2. 1st round of 384 indices introduced by Tn5 tagmentation.
Step 3. 2nd round of 768 indices introduced by hybridization.
Step 4. Gapfilled by T4 DNA polymerase and Ampligase.
Step 5. 3rd round of 96 X N indices introduced by PCR.
Step 6. Next-generation sequencing.