Peptides Bioactive Functional Annotation

Introducing PepAnno

PepAnno is a webserver to annotate the basic information and predict the bioactive function of peptides. It enables the one-click analysis of various physicochemical properties of peptides, while combining multiple machine learning methods for predicting different classes of peptides. Since most of today's machine models have barriers to use after publication, and there are a large number of servers that cannot be used, the creation of PepAnno provides users with a very simple prediction platform.

Go Prediction See Example

The help to use PepAnno

Predict the peptides you're interested in.

First put the peptide sequences you are interested in into the prediction area, make sure they are in fasta format. If you don't know what to do, click the Example button to get the reference input and reference selection, and you can also see an example of the final result through the see example result button. You can compute a peptide profile by amino acid scale. For various categories of scale, you can click to expand and select the scale you are interested in. Hydropathicity Scale of Hydropath Kyte & Doolittle and Transmembrane tendency are selected by default. You can change the window size, which is the length of the interval to use for the profile computation. The default is 9.

Parameter selection of bioactive function & Other optional input.

There are seven kinds of peptide bioactive functions to choose from. You can select all with one click, or you can select individual functions based on your interests. Each function corresponds to some tools to choose from, the default is to use our integrated model options, or you can choose a separate tool to predict according to your interests. For the optional input part, you can input a name for your job. Since the prediction may require waiting, you can also provide an email address, and we will send the results to the email address you provided after the prediction is completed.

Result Visualization - General Information

For the basic information of peptides, we provide a table that allows you to switch between input sequence IDs to display an overview of the data. The table includes details such as the sequence, sequence length, and the predicted biological activity. Additionally, based on the sequence composition, we present two visualizations: a bar chart showing the amino acid composition and a line chart illustrating the amino acid percentage. You can select individual sequences from the legend for focused analysis according to your interests. Icons are also available for selecting and displaying specific information relevant to your research.

Result Visualization - Physical-chemical Information

For the physical-chemical properties of peptides, we provide a table that allows you to switch between input sequence IDs to display an overview of the data. The table includes details such as the sequence, molecular weight, aromaticity, instability index, and isoelectric point. Additionally, we offer line charts illustrating 19 different physical-chemical property types based on sequence composition. You can select individual sequences from the legend for focused analysis according to your interests. Icons are also available for selecting and displaying specific information relevant to your research.

Result Visualization - Structural information

For the structural features of peptides, we provide a table that allows you to switch between input sequence IDs to display an overview of the data. The table includes details such as alpha helix, beta turn, and beta sheet information. Additionally, we use bar charts to show the likelihood of each amino acid position belonging to a specific secondary structure type for each sequence. Tertiary structures are predicted using ESMfold, with visualizations and downloadable PDB links provided. You can select individual sequences from the legend for focused analysis according to your interests.

Result Visualization - Get the bioactive function

For the bioactive functions of peptides, we provide a general results table and a radar chart to display the predicted bioactive functions of all peptides. You can select individual sequences from the legend for focused analysis according to your interests. Additionally, detailed prediction results for each peptide from the selected bioactive prediction tools are presented, along with an integrated summary of possible functions. All prediction results are available for download.