About UUATAC Landscape
We used UUATAC-seq to profile chromatin accessibility landscapes in mouse, chicken, gecko, axolotl, and zebrafish. To allow for public access of the resource, we created a UUATAC Landscape website at http://bis.zju.edu.cn/uuatac/.
UUATAC Landscape website consists of seven web pages.
1. Home: This page contains two sections. About describes the functions and update of the UUATAC Landscape website, workflow gives a brief description of the sequencing method used in the project, UUATAC-seq.
2. Atlas: Explore cell clusters in different datasets. Users can view each cluster, each tissue and each gene by choosing the optional box on the left. Atlas view provides global view on single cell level, Marker list provides marker genes for each cluster.
3. Celltype: Explore detailed information for every cell type in different datasets.
4. Track: Load signal tracks and compare them among cell types/regions in different datasets.
5. Download: This page provide all the datasets information used in this project.
6. FAQ: Some Frequently Asked Questions are listed on this page, inlcuding the corresponding dataset and code address.
7. Contact: Contact information for data providers are placed in this page. We also provide message board on this page.
News
On Mar 18, 2025: Search chromatin accessibility gene score in each celltype and lineage added.
On Mar 10, 2025: Data availability added.
On Dec 18, 2024: Predicted cCREs by NvwaCE and normalized fragments for each subclusters added.
On Oct 31, 2024: UUATAC Landscape released.
Step 1. Nuclei extraction.
Step 2. 1st round of 96 indices introduced by single adaptor Tn5 tagmentation.
Step 3. 2nd round of 768 indices introduced by hybridization & ligation.
Step 4. 3rd round of 8 indices introduced by adapter switch.
Step 5. Gapfilled by T4 DNA polymerase and Ampligase.
Step 6. 3rd round of 96 X N indices introduced by PCR.
Step 7. Next-generation sequencing.